Virus-like particles as vaccines for paramyxovirus

摘要

The invention provides expression vectors and virus-like particles (VLPs) containing Newcastle Disease Virus Sequences in combination with sequences encoding proteins of interest. The vectors are useful in, for example, generating virus-like particles (VLPs) that contain proteins of interest. In one embodiment, the expressed VLPs elicit an immune response by an animal host against the protein. The invention's VLPs are useful as, for example, vaccines.

主权项

1. A non-infectious, replication incompetent, recombinant virus-like particle (VLP) comprising, in operable combination:
a) Newcastle disease virus (NDV) matrix (M) protein, b) NDV nucleocapsid (NP) protein, c) a first chimeric protein comprising NDV haemagglutinin-neuraminidase (HN) protein transmembrane domain (TM) protein flanked by Newcastle Disease Virus (NDV) HN protein cytoplasmic domain (CT) protein and by a first protein sequence that comprises a first copy of a heterologous protein of interest, and d) a second chimeric protein comprising NDV fusion (F) protein transmembrane domain (TM) protein flanked by NDV F protein cytoplasmic domain (CT) protein and by a second protein sequence that comprises a second copy of said heterologous protein of interest,wherein said VLP lacks NDV RNA.