Tissue sealant compositions, vascular closure devices, and uses thereof

摘要

The present invention provides tissue sealant compositions and vasculature closure devices useful for the optical detection of tissue seal and/or clot formation. Compositions and devices of the present invention comprise optical dyes which undergo an observable change as the compositions and/or devices are incorporated into a tissue seal and/or clot, for example a change in fluorescence quantum yield and/or a change in visual color including a change in emission and/or absorption wavelength. Tissue sealants and vasculature closure devices of the present invention are useful for visualizing seal and/or clot formation, for example, during or after surgical procedures, after catheter removal, etc. The present invention further provides methods for formation and optical detection of tissue seals or vasculature puncture closures as well as medical kits useful for the formation and optical detection of tissue seals or vasculature puncture closures.

主权项

1. A tissue sealant comprising:
an adhesive material that is activated to form a seal when contacted with a biological tissue; and an optical dye noncovalently associated with the adhesive material; wherein the optical dye exhibits a first optical condition prior to activation of the adhesive material and exhibits a second optical condition that is distinguishable from the first optical condition after activation of the adhesive material, wherein the first optical condition of the optical dye is a first fluorescence quantum yield prior to activation of the adhesive material and the second optical condition of the optical dye is a second fluorescence quantum yield after activation of the adhesive material, wherein the second fluorescence quantum yield is different from the first fluorescence quantum yield; wherein the first fluorescence quantum yield is substantially equal to 0 and the second fluorescence quantum yield is greater than or equal to 0.01, or wherein the second fluorescence quantum yield is greater than the first fluorescence quantum yield by at least a factor of 1.5 or is less than the first fluorescence quantum yield by at least a factor of 1.5, wherein activation of the adhesive material incorporates the optical dye into the tissue seal, fibrin network, or clot; immobilizes the optical dye in the tissue seal, fibrin network, or clot, or rigidifies the optical dye, thereby providing a change from the first optical condition to the second optical condition; and wherein activation of the adhesive material initiates a coagulation cascade in a subject.