发明名称 |
Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides |
摘要 |
The present invention relates to the field of protein production, and in particular to methods and compositions for modulating glycosylation of proteins expressed in host cells. |
申请公布号 |
US9499614(B2) |
申请公布日期 |
2016.11.22 |
申请号 |
US201414209821 |
申请日期 |
2014.03.13 |
申请人 |
AbbVie Inc. |
发明人 |
Hossler Patrick;McDermott Sean;Racicot Christopher;Correia Ivan |
分类号 |
C07K16/24;C12P21/08;C12P21/00 |
主分类号 |
C07K16/24 |
代理机构 |
McCarter & English, LLP |
代理人 |
McCarter & English, LLP ;Zacharakis Maria Laccotripe |
主权项 |
1. A method of producing a composition comprising an immunoglobulin with an increased level of mannosylated N-glycans and/or a decreased level of fucosylated N-glycans, wherein the immunoglobulin comprises a light chain variable region of SEQ ID NO:1 and a heavy chain variable region of SEQ ID NO:2, said method comprising: culturing a mammalian host cell expressing said immunoglobulin in cell culture media supplemented with 5 mM-100 mM sucrose, thereby producing said composition comprising said immunoglobulin with an increased level of mannosylated N-glycans and/or a decreased level of fucosylated N-glycans as compared to a control, wherein said control is a composition comprising the immunoglobulin produced by culturing the mammalian host cell expressing said immunoglobulin in cell culture media which is not supplemented with sucrose. |
地址 |
North Chicago IL US |