发明名称 |
CELL PROLIFERATION METHOD, AND PHARMACEUTICAL AGENT FOR REPAIR AND REGENERATION OF TISSUE |
摘要 |
Disclosed is a method for proliferating a cell collected from a living body in vitro rapidly and in a large quantity for the purpose of providing a safe and effective pharmaceutical agent for use in the repair/regeneration of a tissue of a living body. Specifically disclosed is a method for culturing a cell in a sample collected from a living body to proliferate the cell. It is preferred to carry out the method in a culture medium which has been determined as being negative for a serum cancer marker and/or an infection factor in advance and which contains a serum from the same species, and to adjust the quantity of an anticoagulant agent (e.g., heparin, a heparin derivative, or a salt of heparin or the heparin derivative) to be added to the collected sample to a quantity less than 5 U/mL of the volume of the sample or adjust the quantity of the anticoagulant agent to a quantity less than 0.5 U/mL in the culture medium before the culture is started. Also disclosed is use of the method. |
申请公布号 |
CA2699236(A1) |
申请公布日期 |
2009.03.19 |
申请号 |
CA20082699236 |
申请日期 |
2008.09.10 |
申请人 |
SAPPORO MEDICAL UNIVERSITY |
发明人 |
HONMOU, OSAMU;HOUKIN, KIYOHIRO |
分类号 |
C12N5/071;A61K35/12;A61K35/28;A61P9/10;A61P25/00;C12N5/07;C12N5/074;C12N5/0775;C12N5/0797 |
主分类号 |
C12N5/071 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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