Surface, anchored Fc-bait antibody display system

摘要

The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. A bait complexed with a monovalent antibody fragment can be expressed on the surface of the host cell wherein the fragment may be assayed for antigen binding while full antibody is simultaneously secreted from the host cell. Methods of using the system for identifying antibodies that bind specifically to an antigen of interest are also provided. Polypeptides, polynucleotides and host cells useful for making the antibody display system are also provided along with methods of use thereof.

主权项

1. An antibody display system comprising an isolated host cell wherein the host cell is selected from the group consisting of a Pichia cell, a Chinese hamster ovary (CHO) cell and a Saccharomyces cerevisiae cell; wherein said isolated host cell comprises
(a) a polynucleotide encoding a bait comprising a heavy Fc immunoglobulin domain fused to a surface anchor polypeptide operably associated with a regulatable promotor; (b) one or more polynucleotides encoding an immunoglobulin light chain variable region; and (c) one or more polynucleotides encoding an immunoglobulin heavy chain variable region wherein the heavy chain is VH—CH1-CH2-CH3.