Endpoint Zygosity Assay To Detect RF4 Gene In Maize

摘要

A method is provided for determining the zygosity of an Rf4 gene in a corn plant. A method may include performing a first PCR assay, a second PCR assay, quantifying probes used in the first and second PCR assays, and comparing the quantified probes to determine zygosity.

主权项

1. A PCR assay method for determining zygosity of an Rf4 gene in a corn plant, the method comprising:
performing a first PCR assay using a first probe, a first forward primer, and a first reverse primer on a polynucleotide from a corn plant sample; performing a second PCR assay using a second probe, a second forward primer, and a second reverse primer on the polynucleotide sample; quantifying the first and second probe; and, comparing the quantified first and second probe to determine zygosity.