System for simultaneous isomerization and fermentation of sugars

摘要

Methods and systems for the isomerization and fermentation of xylose and hexose sugars using an immobilized enzyme system capable of sustaining two different pH microenvironments in a single vessel are disclosed. Bilayer particles are dispersed in a mixture comprising an ionic borate source and xylose. The bilayer particles have a first region with a first enzymatic activity comprising xylose isomerase and a pH of 6 or above, and a second region having a second enzymatic activity at an acidic pH.

主权项

1. A packed bed system comprising:
a packed bed enzyme column having an inlet and an outlet and comprising co-immobilized enzyme particles, the co-immobilized enzyme particles comprising a first region having a first enzymatic activity and a second region having a second enzymatic activity, wherein the co-immobilized enzyme particles are capable of sustaining two different pH microenvironments; and a hollow fiber membrane fermentor having an inlet and an outlet and connected in series to the packed bed enzyme column, the hollow fiber membrane fermentor comprising a microporous hollow fiber membrane composed of microporous hollow fibers, wherein the microporous hollow fiber membrane has a lumen side of the microporous hollow fibers and a shell side of the microporous hollow fibers, the lumen side defining a flow channel and the shell side defining a shell space; wherein the outlet of the packed bed enzyme column is connected directly to the inlet of the hollow fiber membrane fermentor, the inlet being connected to the lumen side of the hollow fiber membrane fermentor.