Inositol Biotransformation

摘要

Disclosed is a method of preparing pure or substantially pure D-myo-inositol-3-phosphate from glucose-6-phosphate and/or fructose-6-phosphate. The method may also be applied to protected and/or derivative forms of glucose-6-phosphate and/or fructose-6-phosphate so as to form protected/derivative forms of D-myo-inositol-3-phosphate, for use in further chemical reactions. The enzyme D-myo-inositol-3-phosphate synthase (INO1) is contacted with the glucose-6-phosphate and/or fructose-6-phosphate to generate labeled or unlabeled, protected or unprotected D-myo-inositol-3-phosphate, which may be further reacted and/or purified.

主权项

1. A method of preparing pure or substantially pure D-myo-inositol-3-phosphate or protected and/or derivative forms thereof, for use in further chemical reactions, comprising:
a) contacting the enzyme D-myo-inositol-3-phosphate synthase (INO1) with labeled or unlabeled, protected or unprotected glucose-6-phosphate and/or fructose-6-phosphate in order to generate labeled or unlabeled, protected or unprotected D-myo-inositol-3-phosphate; and b) optionally further reacting said labeled or unlabeled, protected or unprotected D-myo-inositol-3-phosphate with another enzyme or chemical to generate a further molecule; and c) purifying said labeled or unlabeled, protected or unprotected D-myo-inositol-3-phosphate or said further molecule.