Production of biotin by expressing S-adenosyl-methionine synthase and second biotin synthesis gene in host cells

摘要

Preparation of biotin (I) comprises expressing, in a prokaryotic or eukaryotic host capable of producing (I): (a) an S-adenosyl-methionine synthase sequence (1), of 1155 bp (reproduced); and (b) at least one of the other biotin biosynthesis genes bioS1, 2 or 3 (sequences of 1206 (3), 1215 (5) and 1221 (7) bp, respectively, reproduced). Preparation of biotin (I) comprises expressing, in a prokaryotic or eukaryotic host capable of producing (I): (a) an S-adenosyl-methionine synthase sequence (1), of 1155 bp (reproduced); and (b) at least one of the other biotin biosynthesis genes bioS1, 2 or 3 (sequences of 1206 (3), 1215 (5) and 1221 (7) bp, respectively, reproduced). The transformed cells are cultured and synthesized (I) used directly, after removal of biomass, or after purification. Independent claims are also included for the following: (1) a gene construct containing (1) and at least one of (3), (5) or (7), or their functional variants, analogs or derivatives, linked to either one or more regulatory signals for increased gene and/or protein expression, and/or to their native regulators; (2) organisms containing the construct of (a); and (3) use of the bioS3 gene (7), or its variants, analogs and derivatives, alone or in combination with other biotin synthesis genes, for production of (I).