BHV-1 GENE-DELETED VIRUS VACCINE

摘要

A safe live recombinant virus as well as a vaccine is produced by the deleti on of a portion of the native glycoprotein E(gE) coding region of bovine herpesvirus 1(BHV-1) followed by the insertion of a plasmid including a foreign functional .beta.-gal at the gE locus. The deletion of gE gene stabl y attenuates the virus and serves as an immunological marker differentiating vaccinated animals from infected animals. Moreover, production of .beta.-gal allows easy assessment of gE.DELTA.3.1IBR.beta. virus replication in vaccinated animals and serves as a phenotypic marker which distinguishes it from wild type virus replication in infected animals.