| 发明名称 |
REFOLDING OF POLYPEPTIDES LIKE RECOMBINANT INSULIN-LIKE GROWTH FACTOR IGF-1 |
| 摘要 |
A composition is provided comprising about 0.1 to 15 mg/mL of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40 % (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3 M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9 M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.
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| 申请公布号 |
CA2168552(C) |
申请公布日期 |
2000.02.15 |
| 申请号 |
CA19942168552 |
申请日期 |
1994.08.15 |
| 申请人 |
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发明人 |
BUILDER, STUART;HART, ROGER;LESTER, PHILIP;REIFSNYDER, DAVID |
| 分类号 |
C12P21/02;A61K38/00;C07K1/113;C07K1/14;C07K14/48;C07K14/61;C07K14/65;C12N15/18;C12R1/19;(IPC1-7):C07K14/65;C07K14/475 |
主分类号 |
C12P21/02 |
| 代理机构 |
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代理人 |
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| 主权项 |
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| 地址 |
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