| 摘要 |
An isolated nucleic acid molecule encoding a chimeric membrane protein is provided. The chimeric membrane protein comprises at least two N-terminal transmembrane segments of a prokaryotic membrane protein and the remaining transmembrane segments are eukaryotic. A chimeric membrane protein is also provided. The nucleic acid molecule encoding the chimeric membrane protein can be inserted into a prokaryote, for example, a bacterium such as E. coli. This can be done by introducing a vector into the prokaryote. The chimeric membrane protein encoded by the nucleic acid molecule is then expressed in the prokaryote. |
