| 摘要 |
PURPOSE:To obtain the subject new peptide derived from fish meat, having specific physicochemical characteristics, having angiotensinase-inhibitory activity, thus useful for e.g. hypotensive agents by heating fish meat to deactivate autodigestive enzyme followed by hydrolysis with a protease. CONSTITUTION:Fresh sardine is specifically treated and the meat is taken and ground. The ground meat is then rapidly frozen at <=-30 deg.C followed by grinding with a grinder. Thence, an equal amount of water is added to the resulting meat powder and the mixture is fed to a tank where the mixture is heated at 100 deg.C for 10min to deactivate autodigestive enzyme, thus thermally denaturating the meat powder. The resultant mixture is adjusted in pH value to 10.0 by addition of ammonia water followed by addition of alkali protease to carry out enzymolysis under residence at 45 deg.C for 17hr followed by boiling for 15min to deactivate the enzyme. Thence, the decomposition product is put to gel filtration and purified, thus obtaining the objective neutral new peptide alpha-1000 which has the following characteristics: (1) molecular weight: 200-10000 (determined by column chromatography); (2) decomposable at 119 deg.C, resulting in discoloration; (3) specific rotatory power [alpha]<20>D=-22 deg.; (4) readily soluble to water; and (5) insoluble in ethanol, acetone, and hexane. |
