Crosslinked human or animal tissue products and their methods of manufacture and use

摘要

Degradable bioprostheses made of collagen-based material having amine-based and ester-based crosslinks are provided, as are methods for their formation and use. Some embodiments of the present invention are directed towards a method of controlling the ratio of amine-based crosslinks to ester-based crosslinks within a collagen-based material to provide a tailorably crosslinked collagen-based material. Some embodiments provide a method of making a degradable bioprosthesis involving controlling crosslinking to afford a degradable bioprosthesis that is partially crosslinked. By controlling the ratio of amine-based to ester-based crosslinks, by controlling the level of crosslinking, or by controlling both of these features, degradable bioprostheses with tailored degradation rates can be synthesized. Some embodiments of degradable bioprostheses have degradation rates that are tailored to allow their use in particular medical applications. Some embodiments are directed towards methods of use degradable bioprostheses in wound healing, tissue repair, and tissue supplementation.

主权项

1. A method of treating a tissue defect comprising:
positioning a degradable bioprosthesis at, over, or into the tissue defect, wherein the degradable bioprosthesis comprises: a crosslinked collagen-based material comprising amine-based crosslinks and ester-based crosslinks; wherein the amount of free amines (—NH2) on the collagen based-material is between 50% and 85% relative to the total amount of free amines (—NH2) prior to crosslinking; and wherein the crosslinked collagen based material has a degradation rate between about 0.2% to about 1.0% per hour during the first 24 hours of exposure to a pronase digestion assay.