IMPROVEMENTS TO METHODS FOR PREPARING EQUINE EMBRYOS FOR CRYOPRESERVATION
摘要
<p>A method for preparing an equine embryo for cryopreservation is provided. The method includes collecting an equine embryo with its embryonic capsule. Then after collection the embryo is treated to eliminate or increase the permeability of the capsule in which to enhance subsequent action of cryoprotectant(s). After the treatment the embryo is contacted with cryoprotectant(s) and then placed in a straw with the cryoprotectant(s). Furthermore, the capsule can be treated with enzymes such as collagenase and/or trypsin. Also the embryonic capsule may be removed by mechanical means before subjecting it to the action of cryoprotectant(s). The embryo may also be subjected to an enzymatic treatment to weaken the capsule.</p>
