A PRIMER FOR PCR AMPLIFICATION LINKED WITH COMPLEMENTARY SEQUENCES OR COMPLEMENTARY SEQUENCES INCLUDING MIS-MATCHED NUCLEOTIDES AND NUCLEIC ACID AMPLIFICATION METHOD USING THE SAME

摘要

The present invention relates to a primer for PCR obtained by, directly or by using inosine as a linker, linking a complementary nucleotide sequence or a complementary nucleotide sequence including a mismatched nucleotide sequence to the 5-terminus of a forward or reverse primer; and to a PCR method comprising a step of mixing a nucleic acid template in a PCR composition containing the primer and then performing PCR on the mixture. The primer for PCR of the present invention comprises a complementary nucleotide sequence or a mismatched nucleotide sequence in a complementary nucleotide sequence, which is linked to the 5-terminus thereof directly or via a linker, thereby lowering the sensitivity increase due to the increase in amplification products and reducing non-specifically occurring reactions in PCR.