CUT-OFF ACETYLHYDROLASE OF THROMBOCYTES ACTIVATING FACTOR

摘要

Purified and isolated human plasma platelet- activating factor acetylhydrolase (PAF-AH) polypeptide fragments are new. The polypeptide lacks up to the first twelve N-terminal amino acids of the mature human PAF-AH amino acid sequence of sequence (I) which has 441 amino acids as given in the specification. Independent claims are also included for the following: 1) A variant of the PAF-AH polypeptides which has an amino acid replacement in (I) from the following group: a) S 108 A; b) S 273 A; c) D 286 A; d) D 286 N; e) D 296 A; f) D 304 A; g) D 338 A; h) H 351 A; i) H 395 A; j) H 399 A; k) C 67 S l) C 229 S; m) C 291 S; n) C 334 S; and o) C 407 S. 2) An isolated polynucleotide encoding a PAF-AH polypeptide fragment, variant or variant fragment; 3) A DNA vector containing a DNA which encodes a PAF-AH polypeptide or variant; 4) A host cell stably transformed or transfected with a DNA encoding a PAF-AH polypeptide or variant allowing the expression of the polypeptide; 5) A method of producing a PAF-AH polypeptide fragment or variant by growing a host cell containing the DNA in nutrient and isolating the fragment from the cell or the medium of its growth.