发明名称 |
METHOD FOR LINEAR AMPLIFICATION OF RNA USING HIGH-HEEL PRIMER |
摘要 |
<p>A method for the linear amplification of RNA using a high-heel primer, in which annealing and extension are performed at the same temperature using a high-heel primer. A DNA primer rather than an RNA primer is used, to provide a highly stable linear amplification process that can easily amplify even a small amount of sample, allowing a sufficient amount of target for microarray tests to be obtained from a nanogram level of total RNA by one linear amplification process using a high-heel primer. The inventive method shows a high correlation between RNAs of the same origin regardless of RNA amounts and purification methods, and efficiently achieves linear amplification within a short time at low cost as compared to prior methodology.</p> |
申请公布号 |
KR20060026568(A) |
申请公布日期 |
2006.03.24 |
申请号 |
KR20040075359 |
申请日期 |
2004.09.21 |
申请人 |
GENOMICTREE, INC. |
发明人 |
AN, SUNG WHAN;YOON, CHI WANG;OH, TAE JEONG;YOON, DAE KYOUNG;LEE, SUN WOO;KIM, MYUNG SOON;WOO, SUK KYUNG |
分类号 |
C12N15/10;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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