<p>The expression vectors and methods using an E. coli expression system for the large scale production of FGF18 are described. The vectors utilize the FGF18 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the FGF18 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains, as well as OmpT and fhuA negative strains transformed with an FGF18 expression vector.</p>
申请公布号
WO2006063362(A1)
申请公布日期
2006.06.15
申请号
WO2005US45166
申请日期
2005.12.12
申请人
ZYMOGENETICS, INC.;REARDON, BRIAN, J.;JULIEN, SUSAN, D.;CHAN, CHUNG-LEUNG;LIU, HONG, Y.
发明人
REARDON, BRIAN, J.;JULIEN, SUSAN, D.;CHAN, CHUNG-LEUNG;LIU, HONG, Y.