Expression system

摘要

An immunogenic reagent which produces an immune response which is protective against Bacillus anthracis, said reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) of B. anthracis or variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression from E. coli. High yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.

主权项

1. A recombinant Escherichia coli cell which has been transformed with a heterologous nucleic acid which encodes a protective antigen (PA) of Bacillus anthracis or a variant of the PA of Bacillus anthracis, wherein the percentage of guanine and cytosine residues within the heterologous nucleic acid is in excess of 40%, and wherein the amino acid sequence of PA of Bacillus anthracis or variant thereof is at least 90% identical to amino acid sequence encoded by SEQ ID NO:1.