Methods and compositions for identifying enterovirus

摘要

Certain embodiments of the invention include, but are not limited to PCR primer pairs, sequencing primers, and/or associated thermocycling protocols targeting a region identified by the inventors within the 5′ untranslated region of enteroviruses for the purpose of identifying, subtyping, and/or classifying of virus in samples using nucleic acid sequencing. The sequencing procedures can use nucleic acid templates, such as cDNA or PCR amplicons, as a template for sequencing in medium to high throughput format that is cost effective and easily deployed to other clinical microbiology laboratories.

主权项

1. A method of amplifying enterovirus in a sample comprising:
(a) amplifying a nucleic acid segment from the sample, wherein the nucleic acid segment is an enterovirus 5′UTR corresponding to a nucleic acid segment of rhinovirus A consisting of the nucleic acid sequence of SEQ ID NO:5 to produce an amplicon; and (b) sequencing the amplicon.