Site-specific, covalent bioconjugation of proteins

摘要

Heterofunctional crosslinking groups are provided having the formula: wherein W is a covalent core component; L¹, L² and L³ are independently linking groups; X is a non-covalent or reversibly covalent protein tag binder; Y is a activatable covalent linking group; and Z is a protected or unprotected covalent crosslinking group.