发明名称 |
Methods of performing gene trapping in bacterial and bacteriophage-derived artificial chromosomes and use thereof |
摘要 |
A method of efficiently sequencing multiple exons from complex genomic DNAs is disclosed. The methodology includes the use of bacterial and bacteriophage-derived artificial chromosomes (BBPACs) in novel gene trapping protocols. Targeted gene trapping by homologous recombination, and random gene trapping with the use of a transposon system are exemplified. Included in the invention are methods of preparing a gene map from BBPAC contigs, the resulting gene maps, methods of constructing a cDNA library from BBPAC contigs, and the resulting cDNA libraries.
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申请公布号 |
US6156574(A) |
申请公布日期 |
2000.12.05 |
申请号 |
US19980102488 |
申请日期 |
1998.06.22 |
申请人 |
THE ROCKEFELLER UNIVERSITY |
发明人 |
HEINTZ, NATHANIEL;JIANG, WEINING;YANG, XIANGDONG W. |
分类号 |
A01K67/027;C12N15/10;C12N15/70;C12N15/90;(IPC1-7):C12N15/87 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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