Ketol-acid reductoisomerase enzymes and methods of use

摘要

Provided herein are polypeptides having ketol-acid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.

主权项

1. A polypeptide having ketol-acid reductoisomerase activity, wherein the polypeptide comprises
a. the amino acid sequence of SEQ ID NO: 42; b. at least 99% identity to SEQ ID NO: 42; c. at least 99% identity to SEQ ID NO: 42 and at least one of the following substitutions: M301I, Y239H, Y113F, S322A, A71K, N114G, A329R, A69T, N114S, G72W, A295V, E264K, R280D, A329E, S157T, M238I, Q272T, K335M, R280H, and a combination thereof; or d. an active fragment of any one of (a) to (c).