ENZYMATIC SYSTEMS FOR CARBON FIXATION AND METHODS OF GENERATING SAME

摘要

A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.

主权项

1. A system for carbon fixation, comprising an electron donor and enzymes which catalyze reactions of a carbon fixation pathway, wherein all the carboxylation reactions of the carbon fixation pathway utilize:
(i) phophoenolpyruvate (PEP) carboxylase and acetyl-CoA carboxylase; or (ii) phophoenolpyruvate (PEP) carboxylase; or (iii) pyruvate carboxylase and acetyl-CoA carboxylase; or (iv) pyruvate carboxylase wherein products of said reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, and wherein an additional product of the carbon fixation pathway is glyoxylate, wherein an in-organic carbon is introduced into a substrate to become a carboxylic acid group during said carboylation reactions.