RECOMBINANT VACCINES COMPRISING IMMUNOGENIC ATTENUATED BACTERIA HAVING RPOS POSITIVE PHENOTYPE

摘要

Attenuated immunogenic bacteria having an RpoS<SUP>+</SUP> phenotype, in particular, Salmonella enterica serotype Typhi having an RpoS<SUP>+</SUP> phenotype and methods therefor are disclosed. The Salmonella have in addition to an RpoS<SUP>+</SUP> phenotype, an inactivating mutation in one or more genes which render the microbe attenuated, and a recombinant gene capable of expressing a desired protein. The Salmonella are attenuated and have high immunogenicity so that they can be used in vaccines and as delivery vehicles for genes and gene products. Also disclosed are methods for preparing the vaccine delivery vehicles.