Functionalized microfluidic device for immunofluorescence

摘要

It is described a microfluidic device, for use in the field of analytical fluorescence based assays and, in particular, in FISH assays.

主权项

1. A microfluidic device for visual analysis of immobilized cells comprising a slide and a part in which at least one groove is present, said part being a pad of silicone and being reversibly coupled to the slide due to spontaneous adhesion between said pad and said slide, said slide and said part being such that their joining defines a microchannel, said microchannel being accessible from a top surface of the device via through holes present in said part, wherein at least the area on a surface of said slide facing said microchannel is functionalized with a hydrophilic nanostructured metal oxide film of thickness comprised between 20 nm and 200 nm with a wettability contact angle lower than 10°, wherein the nanostructured metal oxide film has a porous structure with a mass density from about ½ to about 1/10 of the mass density of the bulk oxide corresponding to the nanostructured metal oxide film.