发明名称 |
mutantes de sulfidrilase o-fosfoserina e um método para produção da cisteína usando o mesmo |
摘要 |
Disclosed is an O-phosphoserine sulfhydrylase (OPSS) mutant which has a Mycobacterium smegmatis -derived amino acid sequence corresponding to that of SEQ ID NO: 1 which is devoid of three to seven C-terminal amino acid residues. Also, a nucleic acid molecule encoding the OPSS mutant, an expression vector carrying the nucleic acid molecule, and a transformant transformed with the expression vector are disclosed. In addition, a method is provided for producing cysteine in which O-phospho-L-serine (OPS) is reacted with a sulfide in the presence of the OPSS mutant. The OPSS mutant has improved enzymatic activity and can be applied to the environmentally friendly production of L-cysteine through a simple enzymatic conversion reaction. |
申请公布号 |
BR112013009633(A2) |
申请公布日期 |
2016.07.19 |
申请号 |
BR20131109633 |
申请日期 |
2011.10.14 |
申请人 |
CJ CHEILJEDANG CORPORATION |
发明人 |
BYEONG CHEOL SONG;HYE WON UM;JAE HYUN JO;JIN SOOK CHANG;KYOUNG MIN LEE;SOO AN SHIN |
分类号 |
C12N9/10;C12N15/54;C12P13/12 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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