| 摘要 |
<p>The present invention relates to nucleoprotein (NP) genes of the influenza A and B viruses which were constructed from virion RNA and subsequently expressed in Spodoptera frugiperda (S19) cells using the baculovirus vector, Autographa californica nuclear polyhedrosis virus (AcNPV). The baculovirus-expressed NP antigens were reactive with monoclonal antibodies specific for either type A or B NP and with anti-NP antibodies in human serum samples. The expressed influenza NP antigens comigrated in electrophoresis with NP purified from influenza A or B virions and the recombinant NP antigens represented greater than 10 % of total protein in infected cells. Dilutions of clarified S19 cell lysates were used as antigens in a standard enzyme immunoassay format to detect serum antibody specific for influenza A or B viruses. The results showed that baculovirus-expressed NP antigens can be used in reproducible and routine assays for the serodiagnosis of influenza virus infections.</p> |
