发明名称 |
Recombinant DNA clone containing a genomic fragment of PfHRP-II gene from Plasmodium falciparum |
摘要 |
The invention relates to isolated clones of DNA from Plasmodium falciparum that encode a histidine-rich protein from that organism. The PfHRPII protein is expressed in P. falciparum-infected erythrocytes. The cloned gene segment includes an intron-exon boundary near the amino-terminus of the coding sequence. The PfHRPII protein has a Mr of 60-80 kDa as determined by SDS-PAGE. This is substantially higher than the molecular weight of about 35 kDa as estimated from the predicted amino acid sequence of PfHRPII. The PfHRPII amino acid sequence includes a hydrophobic leader sequence, consistent with secretion of PfHRPII observed in vivo and in vivo. The amino acid sequence of PfHRPII is also characterized by a number of tandem repeats having a high content of histidine, alanine and aspartic acid.
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申请公布号 |
US5476785(A) |
申请公布日期 |
1995.12.19 |
申请号 |
US19930161406 |
申请日期 |
1993.12.06 |
申请人 |
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE DEPARTMENT OF HEALTH AND HUMAN SERVICES |
发明人 |
WELLEMS, THOMAS E.;HOWARD, RUSSELL J. |
分类号 |
A61K39/00;C07K14/445;(IPC1-7):C12N1/21;C12N15/30;C12N15/70 |
主分类号 |
A61K39/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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