NACHWEISVERFAHREN FÜR EINEN LIGANDEN DER SELEKTIV EINE FUNKTIONELLE KASKADE MODULIEREN KANN UND EIN TARGET IMPLIZIERT SOWIE DESSEN VERWENDUNG FÜR HOCHDURCHSATZ-SCREENING VON MOLEKÜLEN DIE VON INTERESSE SIND

摘要

The invention concerns a method for identifying a ligand capable of selectively modulating a functional cascade involving a target, and uses thereof for high-throughput screening of molecules of interest, in particular therapeutic (medicine). Said method comprises at least the following steps: a) identifying an antibody or an antibody fragment comprising at least one of the variable domains of an immunoglobulin chain, capable of binding to said target and modulating said functional cascade involving said target; b) screening from a bank of molecules, ligands modulating the bond between said target and the antibody or antibody fragment identified in a); and c) identifying among said modulating ligands obtained in b), those capable of modulating said functional cascade.