Soybean seed and oil compositions and methods of making same

摘要

Methods for obtaining soybean plants that produce seed with low linolenic acid levels and moderately increased oleic levels are disclosed. Also disclosed are methods for producing seed with low linolenic acid levels, moderately increased oleic levels and low saturated fatty acid levels. These methods entail the combination of transgenes that provide moderate oleic acid levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. These methods also entail the combination of transgenes that provide both moderate oleic acid levels and low saturated fat levels with soybean germplasm that contains mutations in soybean genes that confer low linolenic acid phenotypes. Soybean plants and seeds produced by these methods are also disclosed.

主权项

1. A method of producing soybean seed comprising a linolenic acid content of 0.5% to 3% of total seed fatty acids by weight, a saturated fatty acid content of 10% to 15% of total seed fatty acids by weight, and an oleic acid content of 70% to 79% of total seed fatty acids by weight, comprising the steps of:
a) growing one or more soybean plants that comprise a transgene that decreases the expression of an endogenous soybean FAD2-1 gene and at least two loss-of-function mutations in at least two endogenous soybean FAD3 genes, wherein said one or more soybean plants yield seed having a seed fatty acid composition comprising a linolenic acid content of 0.5% to 3% of total seed fatty acids by weight, a saturated fatty acid content of 10% to 15% of total seed fatty acids by weight, and an oleic acid content of 70% to 79% of total seed fatty acids by weight; and b) harvesting seed from said one or more soybean plants thereby producing the soybean seed.