COMPOSITIONS FOR RNA-CHROMATIN INTERACTION ANALYSIS AND USES THEREOF

摘要

The invention described herein provides reagents (e.g., kits), compositions, and methods for carrying out an unbiased genome-wide strategy to identify the functional targets for all ncRNAs.

主权项

1. A kit comprising:
(1) an RNA linker comprising:
(i) a first polynucleotide, and,(ii) a second polynucleotide,wherein the first and the second polynucleotides form a first double stranded region flanked by a first ligation compatible end, and a 3′-overhang at the 3′-end of the first polynucleotide, wherein the 3′-overhang comprises a random-sequence primer; and, (2) a DNA linker comprising:
(iii) a third polynucleotide, and,(iv) a fourth polynucleotide,wherein the third and the fourth polynucleotides form a second double stranded region flanked by a blunt end and a second ligation compatible end, wherein the first and the second ligation compatible ends ligate to each other, or are adaptable to ligate to each other.