| 发明名称 |
Methods and systems for streamlining optical calibration |
| 摘要 |
A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array. |
| 申请公布号 |
US9495741(B2) |
申请公布日期 |
2016.11.15 |
| 申请号 |
US201214348206 |
申请日期 |
2012.09.30 |
| 申请人 |
LIFE TECHNOLOGIES CORPORATION |
发明人 |
Freudenthal Jacob K.;Marks Jeffrey A.;Cheng Francis T.;Wessel Thomas |
| 分类号 |
G06K9/00;G06T7/00;G06T5/00;G06T5/50 |
主分类号 |
G06K9/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for calibrating a biological instrument comprising:
executing a pre-scan calibration comprising:
acquiring an image of at least one biological sample array;evaluating the image to determine fluorescence levels;modifying the fluorescence levels by adjusting an excitation source with an exposure time and a duty cycle to provide strong fluorescence levels without saturation; determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array. |
| 地址 |
Carlsbad CA US |
