METHOD FOR PERFORMANCE OPTIMIZATION OF PROTEIN CHIP PRODUCED UNDER EXTERNAL ELECTRIC FIELD APPLIED IN DIFFERENT DIRECTION AND DEVICE FOR PROVIDING EXTERNAL ELECTRIC FIELD IN DIFFERENT DIRECTION

摘要

A method for performance optimization of protein chips produced under an external electric field applied in different directions and a device that provides the external electric field in different directions are revealed. Firstly a plurality of protein chips is produced under an external electric field applied in different directions. Then a binding force between protein molecule on the protein chip and a ligand is measured and compared. Thus an angle of the external electric field applied that achieves performance optimization while using the protein molecule to produce the protein chips is found out. The device providing the external electric field in different directions includes a rotatable electric field support rotating around a carrier used for loading the protein chips. The electric field support is disposed with electrodes for providing the protein chips on the carrier the external electric field in different directions.

主权项

1. A method for performance optimization of protein chips produced under an external electric field applied in different directions comprising the steps of:
taking and dropping a protein solution containing at least one protein molecule to a first chip; applying an external electric field to the first chip for deflecting and fixing the protein molecule on the first chip while an angle between the external electric field and a line perpendicular to the first chip is a first angle; taking and dropping the protein solution to a second chip; applying an external electric field to the second chip for deflecting and fixing the protein molecule on the second chip while the angle between the external electric field and a line perpendicular to the second chip is a second angle; measuring a first binding force between at least one ligand molecule for the protein molecule and the first chip, as well as a second binding force between the ligand molecule for the protein molecule and the second chip; and comparing the first binding force with the second binding force to determine the angle of the external electric field applied that achieves performance optimization of the protein chips while using the protein molecule to produce the protein chips; the angle of the external electric field applied is selected from the group consisting of the first angel and the second angle.