Treating bladder tumor cells using fibronectin attachment protein as a target

摘要

Composition and methods are disclosed for utilizing micro-aggregation of FAP-containing complexes to promote their fast internalization. This approach allows the uptake of cytotoxic cargo coupled to either FAP-Antibodies or FAP-liposome complexes by tumor bladder cells. Importantly, this approach is efficient even under serum-free conditions such as the ones found in the lumen of the bladder.

主权项

1. A method of enhancing targeted uptake of therapeutic or diagnostic agents by a patient's cells, said method comprising the steps of:
providing a multivalent complex comprising at least one carrier and a plurality of fibronectin attachment proteins or functional equivalents thereof attached to the carrier such that each fibronectin attachment protein or functional equivalent thereof is positioned within submicrons of at least one other fibronectin attachment protein or functional equivalent to form a cluster and wherein said complex is linked to a therapeutic or diagnostic agent; and administering said complex to said patient; wherein upon contact between receptors of a patient cell and the fibronectin attachment proteins or functional equivalents thereof of the complex, the cluster further comprises the receptors of the patient cell.