ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC FEEDSTOCKS USING ACCESSORY ENZYMES

摘要

Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.09 to about 0.91 (w/w), Swollenin at a fractional concentration (fS wo1) of about 0 to about 0.91(w/w), and Cip1 at a fractional concentration (fCip1) of 0 to about 0.42 measured relative to all of the accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.