Cell culture of fetal liver in layered state in a partitioned micro-space

摘要

Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.

主权项

1. A cell culture method comprising:
culturing fetal liver cells in a layered state in a partitioned micro-space, thereby obtaining a cell mass containing differentiated liver parenchymal cells, wherein the fetal liver cells differentiates in the partitioned micro-space, and the fetal liver cells are at least one of fetal liver stem cells and fetal liver precursor cells.