Device and method for amplifying target nucleic acid

摘要

A method for amplifying a target nucleic acid present in a sample includes introducing, via an entrance opening of a planar fluidic assembly, a sample containing one or more target nucleic acids into a flow channel of the planar fluidic assembly, the flow channel extending from the entrance opening to an exit vent and having a substantially uniform cross-section. A plurality of nucleic acid primers that are complementary to a portion of the one or more target nucleic acids are disposed at locations within and along the flow channel. The method further includes subjecting the sample introduced into the flow channel to a primer-based amplification reaction using the nucleic acid primers, wherein the primer-based amplification reaction produces amplified product of the one or more target nucleic acids, and retaining the amplified product at one or more of the locations within the flow channel during the primer-based amplification reaction. The method also includes detecting localized accumulation of the retained amplified product, the localized accumulation occurring at the one or more locations.

主权项

1. A method for amplifying a target nucleic acid present in a sample, the method comprising:
introducing, via an entrance opening of a planar fluidic assembly, a sample containing one or more target nucleic acids into a flow channel of the planar fluidic assembly, the flow channel extending from the entrance opening to an exit vent, the flow channel having a substantially uniform cross-section from the entrance opening to the exit vent, wherein a plurality of nucleic acid primers that are complementary to a portion of the one or more target nucleic acids are preloaded and retained at locations within and along the flow channel from the entrance opening to the exit vent; subjecting the sample introduced into the flow channel to a primer-based amplification reaction using the nucleic acid primers, wherein the primer-based amplification reaction produces amplified product of the one or more target nucleic acids; retaining the amplified product at one or more of the locations within the flow channel during the primer-based amplification reaction; and detecting localized accumulation of the retained amplified product, the localized accumulation occurring at the one or more locations.