发明名称 |
HIGH-THROUGHPUT ASSAY FOR VIRUS ENTRY AND DRUG SCREENING |
摘要 |
The present invention provides a rapid virus entry/binding detection assay. An enzyme such as luciferase was incorporated at the C-terminal end of viral envelope proteins that would deliver the enzyme into the viral particles upon viral assembly. Virus entry/binding can then be assayed by determining the enzymatic activities in infected cells. The assay allows high-throughput non-radioactive detection of virus entry within 30 minutes after virus-cell contact. This assay provides high signal to noise ratio and is useful for screening compounds that affect virus-cell binding and entry. The design also permits packaging of potential therapeutic proteins into functional virus particles and delivering them to specific cellular targets. |
申请公布号 |
WO2004089310(A3) |
申请公布日期 |
2007.09.20 |
申请号 |
WO2004US09868 |
申请日期 |
2004.03.30 |
申请人 |
THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
DAVEY, ROBERT, A.;KOLOKOLTSOV, ANDREY |
分类号 |
C07K14/15;C12Q1/37;C12Q1/66;G01N33/569 |
主分类号 |
C07K14/15 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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