Method for determination of a component of a sample.

摘要

This invention relates to a method for determining a component of a test sample with a solid support, said support having incorporated therein a removable first monoclonal antibody which binds to said component and a labeled monoclonal antibody or fragment of a monoclonal antibody which binds to said component wherein said first monoclonal antibody and said labeled monoclonal antibody or monoclonal antibody fragment are derived from the same species under conditions favoring formation of a complex of said first monoclonal antibody said component and said labeled monoclonal antibody or fragment, contacting said complex with an immobilized second receptor which binds to said first monoclonal antibody but not to said labeled monoclonal antibody or fragment under conditions favoring formation of a quaternary complex between said first complex and said immobilized receptor, and measuring label either in said quaternary complex or in a residue of said sample as a measure of said component.