COMPOSITIONS AND METHODS OF NUCLEIC ACID PREPARATION AND ANALYSES

摘要

The present invention provides methods of generating single-stranded polynucleotides comprising use of adaptor sequence(s), single-stranded polynucleotide amplification and a primer comprising BJSA. Methods of analysing one or more regions on a desired polynucleotide using probes and single-stranded polynucleotides are also provided. Also provided are kits and compositions useful for these methods.