Recovering microorganisms from soil by suspending soil in a citrate buffer

摘要

Microorganisms are recovered from soil by suspending a soil sample containing the microorganisms in a buffer solution comprising an organic acid of 3 or more carbon atoms, and then separating the microorganisms into supernatant of the suspension. Furthermore, the citrate buffer is a citric acid and a salt of citric acid. Also microorganisms are separated from the soil particles and recovered from the suspension by centrifugation or electrophoresis.