摘要 |
In a chromatography using a pressurized mobile phase, the mobile phase flowing through a feed pipe (31) is introduced into one end of a bypass pipe (32) where a sample is sealed through a two-way valve (v2), a two-way valve (v3) is opened and a two- way valve (v1) is closed when the sample is pressurized by the introduction of the mobile phase, all the pressurized sample is thereby injected into the feed pipe (31), and further the pressure variation of the mobile phase in the feed pipe is suppressed when the sample is injected.
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