Monoclonal antibody for detecting exosomes

摘要

A monoclonal antibody for detecting or capturing an exosome, selected from the group consisting of a monoclonal antibody or antibody fragments thereof that recognize amino acid numbers 113 to 195 of the amino acid sequence as shown in SEQ ID NO: 1, a monoclonal antibody or antibody fragments thereof that recognize amino acid numbers 104 to 202 of the amino acid sequence as shown in SEQ ID NO: 2, a monoclonal antibody or antibody fragments thereof that recognize amino acid numbers 36 to 54 of the amino acid sequence as shown in SEQ ID NO: 3, and a monoclonal antibody or antibody fragments thereof that recognize amino acid numbers 113 to 201 of the amino acid sequence as shown in SEQ ID NO: 3, each capable of detecting or capturing an exosome. The monoclonal antibody for detecting an exosome of the present invention is capable of detecting CD9, CD63 or CD81 on the exosome in a living body with an excellent sensitivity and specificity.

主权项

1. A monoclonal antibody for detecting or capturing an exosome selected from the group consisting of:
a monoclonal antibody (CD9-12A12 antibody) produced by a hybridoma deposited under Accession Number FERM BP-11519 or fragments thereof which are monoclonal antibody fragments that recognize amino acid numbers 113 to 195 of the amino acid sequence as shown in SEQ ID NO: 1; a monoclonal antibody (CD63-8A12 antibody) produced by a hybridoma deposited under Accession Number FERM BP-11520 or fragments thereof which are monoclonal antibody fragments that recognize amino acid numbers 104 to 202 of the amino acid sequence as shown in SEQ ID NO: 2; a monoclonal antibody (CD63-13C8 antibody) produced by a hybridoma deposited under Accession Number FERM BP-11521 or fragments thereof which are monoclonal antibody fragments that recognize amino acid numbers 104 to 202 of the amino acid sequence as shown in SEQ ID NO: 2; a monoclonal antibody (CD81-4G6 antibody) produced by a hybridoma deposited under Accession Number NITE BP-1480 or fragments thereof which are monoclonal antibody fragments that recognize amino acid numbers 113 to 201 of the amino acid sequence as shown in SEQ ID NO: 3; a monoclonal antibody (CD81-6D12 antibody) produced by a hybridoma deposited under Accession Number NITE BP-1481 or fragments thereof which are monoclonal antibody fragments that recognize amino acid numbers 113 to 201 of the amino acid sequence as shown in SEQ ID NO: 3; and a monoclonal antibody (CD81-12C4 antibody) produced by a hybridoma deposited under Accession Number NITE BP-1482 or fragments thereof which are monoclonal antibody fragments that recognize amino acid numbers 36 to 54 of the amino acid sequence as shown in SEQ ID NO: 3.