EFFICIENT BIOMOLECULE RECYCLING METHOD AND SYSTEM

摘要

A technique is disclosed for recapturing and recycling biomolecule reagents. The technique may be applied in a range of settings, including biopolymer synthesis, sequencing, and so forth. Biomolecule reagents such as nucleotides and oligonucleotides used to process nucleic acids, which may be marked with fluorescent tags, carry blocking agents, and so forth, are introduced to samples in a sample container. After the desired reaction occurs with some of the biomolecule reagents, such as some of the nucleotides or oligonucleotides, the effluent stream is processed to recapture unreacted biomolecule reagents. These may be separated from other reaction components, and recycled into the same or a different sample container. The recaptured biomolecule reagents may be mixed with additional biomolecule reagents prior to reintroduction to the same or different samples.

主权项

1. A method for processing a biopolymer sample comprising:
introducing a process fluid containing biomolecule reagents to a sample container in which a portion of the biomolecule reagents modify a biopolymer sample in the container; processing an effluent stream of the process fluid flowing from the sample container to recapture biomolecule reagents in the effluent stream that are present after the biopolymer sample is modified; and introducing the recaptured biomolecule reagents into the same or a different sample container under conditions for modifying a biopolymer sample with the recaptured biomolecule reagents.