Plastidial nucleotide sugar epimerases

摘要

Isolated polynucleotides and polypeptides and recombinant DNA constructs of plastidial sugar epimerases useful for conferring improved agronomic performance including yield and drought are disclosed. Compositions (such as plants or seeds) having these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs are also disclosed.

主权项

1. A method of increasing the production of UDP-galactose in situ in the chloroplast for mono-galactosyldiacylglycerol (MGDG) biosynthesis in a plant, the method comprising expressing from a DNA construct comprising a nucleic acid encoding a chloroplast UDP-glucose epimerase under the control of at least one regulatory promoter element in the plant, wherein the chloroplast UDP-glucose epimerase comprises a chloroplast transit peptide, and wherein the UDP-glucose epimerase is a monocot polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO: 1.