MICROPLATE-BASED APPARATUS FOR MEASURING CELL METABOLISM

摘要

A microplate-based apparatus for measuring cell metabolism is provided. The apparatus is capable of providing automation of all procedures, ranging from drug injection into each well of a microplate to the measurement of dissolved-oxygen (DO) concentration and hydrogen ion concentration.

主权项

1. An apparatus for measuring cell metabolism, comprising:
an X-Y DRIVING stage table movable horizontally and vertically; a microplate which comprises a plurality of wells and is mounted on a top of the X-Y DRIVING stage table, each of the wells accommodating a cell and comprising a dissolved oxygen (DO) sensing film and a hydrogen ion concentration (pH) sensing film; a sensor board which is situated on the top of the X-Y DRIVING stage table and comprises pairs of a light emitting element for DO detection and a light detecting element for DO detection, each pair vertically corresponding to the DO sensing film in each well of the microplate mounted on the X-Y DRIVING stage table, and pairs of a light emitting element for pH detection and a light detecting element for pH detection, each pair vertically corresponding to the pH sensing film in each well; a plurality of cartridges containing drugs; a plurality of infusion pumps disposed at fixed positions and configured to inject the drugs supplied from the cartridges through nozzles to the wells of the microplate; and a controller configured to control movement of the X-Y DRIVING stage table and measure DO concentration and a pH with respect to at least one well of the microplate.