Genetically stable oncolytic RNA virus, method of manufacturing and use thereof

摘要

In a method for manufacturing a modified enterovirus of ECHO 7 type by modification of native ECHO 7 virus, isolated by a known method from human feces and identified by genome sequence, the modification is performed initially conducting the virus adaptation in cancer cells, attenuated by anti-cancer agent dacarbazine, further passaging the modified virus in human embryonal fibroblast culture, followed by propagation in human melanoma cells and further passaging in human embryonal fibroblast culture, that was treated by ribavirin, isolation and purification by known method. The modified virus is suitable for treating various tumours.

主权项

1. Modified enterovirus of ECHO 7 type characterized by stable genome sequence that has at least about 85%, preferably at least about 95%, still more preferably at least about 99% of sequence identical to Seq ID No 1.