Electroporation devices and methods of using same for electroporation of cells in mammals

摘要

Aspects of the present invention relate to electroporation devices and methods of using same to effectively facilitate the introduction of a biomolecule into cells of a selected tissue in a body, in particular skin such as intradermic or subcutaneous tissue. In some aspects, the present invention is a skin EP device, which produces a pulse of energy and delivers same to the skin tissue using a skin electrode array and maintains a constant current in the same skin tissue based on user input, including a preset current, and allows the storage and acquisition of current waveform data.

主权项

1. An electroporation device configured to deliver to a desired tissue of a mammal a pulse of energy producing a constant current similar to a preset current input by a user, wherein the desired tissue is subcutaneous tissue or intradermic tissue, the electroporation device comprising:
an electroporation component capable of delivering the pulse of energy that produces the constant current in the desired tissue, the electroporation component having a feedback mechanism; and a skin electrode assembly including an electrode array having a plurality of skin electrodes in a spatial arrangement, wherein the electrode array comprises at least three skin electrodes, wherein the skin electrodes are needle electrodes, and wherein the skin electrode assembly receives the pulse of energy from the electroporation component and delivers same to the desired tissue through the skin electrodes, wherein at least one of the plurality of skin electrodes is neutral during delivery of the pulse of energy and measures impedance in the desired tissue and communicates said impedance to the electroporation component, wherein the feedback mechanism can receive the measured impedance and can adjust the pulse of energy delivered by the electroporation component to maintain the constant current, wherein the feedback mechanism is performed by an analog closed-loop circuit, wherein the neutral electrode measures the impedance in the desired tissue and communicates the impedance to the feedback mechanism, and the feedback mechanism responds to the impedance and adjusts the pulse of energy to maintain the constant current at a value similar to the preset current, and wherein the feedback mechanism maintains the constant current continuously and instantaneously during the delivery of the pulse of energy.